Hotspot

trnV-UAC-trnM-CAU

LSC · 45810-47126 · 1317 bp

Back to Codonopsis

Pi 0.0229

MarkerSeek score 73.40

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1317
variable_sites: 87
pi: 0.0229
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	TACCCACTGAGCTATAGCCC	GTATTGCTTTCATACGGCGG	55.0	50.0	58.0	57.3	1376-2417 bp	1.0	81.1
trnV-UAC-trnM-CAU_p2	AGTAAGACGGGGAAAGGTGA	GTATTGCTTTCATACGGCGG	50.0	50.0	58.0	57.3	1459-2500 bp	1.0	80.8
trnV-UAC-trnM-CAU_p3	AAGGGCCCCTTTGAAAAACA	GTATTGCTTTCATACGGCGG	45.0	50.0	58.1	57.3	1422-2463 bp	1.0	80.1
trnV-UAC-trnM-CAU_p4	AGGGCCCCTTTGAAAAACAT	GTATTGCTTTCATACGGCGG	45.0	50.0	57.9	57.3	1421-2462 bp	1.0	80.1
trnV-UAC-trnM-CAU_p5	TGAAGGGCCCCTTTGAAAAA	GTATTGCTTTCATACGGCGG	45.0	50.0	57.8	57.3	1424-2465 bp	1.0	79.6