Hotspot

rps2

Genome · 59928-60617 · 690 bp

Back to Codium

Pi 0.158
MarkerSeek score 82.50
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
Genome
length_bp
690
variable_sites
183
pi
0.158
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 AGGGGTACGGGAACAAGTAA GAACTAGTGACATTCCGCGT 50.0 50.0 58.0 58.0 1939 bp 0.25 49.7
rps2_p2 TTAGGGGTACGGGAACAAGT GAACTAGTGACATTCCGCGT 50.0 50.0 58.0 58.0 1941 bp 0.25 49.7
rps2_p3 AGGGGTACGGGAACAAGTAA TGAACTAGTGACATTCCGCG 50.0 50.0 58.0 58.0 1940 bp 0.25 49.7
rps2_p4 TTAGGGGTACGGGAACAAGT TGAACTAGTGACATTCCGCG 50.0 50.0 58.0 58.0 1942 bp 0.25 49.7
rps2_p5 AGGGGTACGGGAACAAGTAA CCCGGGGGTATTTTTAGAGC 50.0 55.0 58.0 58.0 1497 bp 0.25 49.6