Hotspot

rpl5

Genome · 68243-69069 · 827 bp

Back to Codium

Pi 0.195
MarkerSeek score 83.60
Primer available yes
Label rpl5

Feature Metadata

label_name
rpl5
feature_type
gene
region
Genome
length_bp
827
variable_sites
256
pi
0.195
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl5_p1 ATAAATGCGTCGTCCTGGTG TTTCGGACCTATTGCTCGTG 50.0 50.0 58.3 58.0 1200 bp 0.25 47.2
rpl5_p2 ATAAATGCGTCGTCCTGGTG AATTACAAAACGTGCCGCTG 50.0 45.0 58.3 58.0 1712 bp 0.25 46.9
rpl5_p3 ATAAATGCGTCGTCCTGGTG TTGGTTAACCGCTCGACAAA 50.0 45.0 58.3 58.0 1811 bp 0.25 46.8
rpl5_p4 CGTCCTGGTGTACTGATACG TTTCGGACCTATTGCTCGTG 55.0 50.0 57.5 58.0 1190 bp 0.25 46.2
rpl5_p5 ATAAATGCGTCGTCCTGGTG TTGCAGTTGTCAAACCAGGT 50.0 45.0 58.3 58.2 1650 bp 0.25 46.0