Hotspot

rbcL

Genome · 52476-55624 · 3149 bp

Back to Codium

Pi 0.19
MarkerSeek score 79.20
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
Genome
length_bp
3149
variable_sites
940
pi
0.19
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 CTCCGGTAAGATAGTCGGGT CGTATGACTCCACAACCTGG 55.0 55.0 58.0 58.0 2418-2464 bp 0.5 70.2
rbcL_p2 CTAGTGTTAGCGGTCCAACC AACATTTCAAGGACCACCCC 55.0 50.0 58.0 58.0 2120 bp 0.25 49.9
rbcL_p3 CTAGTGTTAGCGGTCCAACC CGTATGACTCCACAACCTGG 55.0 55.0 58.0 58.0 2437 bp 0.25 49.9
rbcL_p4 ACCTCCGGTAAGATAGTCGG AACATTTCAAGGACCACCCC 55.0 50.0 58.0 58.0 2103 bp 0.25 49.8
rbcL_p5 CTCCGGTAAGATAGTCGGGT AACATTTCAAGGACCACCCC 55.0 50.0 58.0 58.0 2101 bp 0.25 49.8