Hotspot

psbB

Genome · 30208-32538 · 2331 bp

Back to Codium

Pi 0.152
MarkerSeek score 82.20
Primer available yes
Label psbB

Feature Metadata

label_name
psbB
feature_type
gene
region
Genome
length_bp
2331
variable_sites
558
pi
0.152
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB_p1 TGTCCATAGAGTGCTTGTGT GCCTGGAGTGACTTTTCCAT 45.0 50.0 56.5 57.8 2940 bp 0.25 36.2
psbB_p2 TGTCCATAGAGTGCTTGTGT AAAAGTGTAGTTCCCCAGCC 45.0 50.0 56.5 57.7 2957 bp 0.25 35.5
psbB_p3 TGTCCATAGAGTGCTTGTGTT GCCTGGAGTGACTTTTCCAT 42.9 50.0 57.2 57.8 2940 bp 0.25 34.2
psbB_p4 TTGTCCATAGAGTGCTTGTGT GCCTGGAGTGACTTTTCCAT 42.9 50.0 57.2 57.8 2941 bp 0.25 34.2
psbB_p5 TGTCCATAGAGTGCTTGTGT CAGCCTGGAGTGACTTTTCC 45.0 55.0 56.5 58.5 2942 bp 0.25 34.0