Hotspot

orf276

Genome · 53178-54008 · 831 bp

Back to Codium

Pi 0.296
MarkerSeek score 84.10
Primer available yes
Label orf276

Feature Metadata

label_name
orf276
feature_type
gene
region
Genome
length_bp
831
variable_sites
384
pi
0.296
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf276_p1 CTCCGGTAAGATAGTCGGGT CGTATGACTCCACAACCTGG 55.0 55.0 58.0 58.0 2418-2464 bp 0.5 70.2
orf276_p2 CTAGTGTTAGCGGTCCAACC AACATTTCAAGGACCACCCC 55.0 50.0 58.0 58.0 2120 bp 0.25 49.9
orf276_p3 CTAGTGTTAGCGGTCCAACC CGTATGACTCCACAACCTGG 55.0 55.0 58.0 58.0 2437 bp 0.25 49.9
orf276_p4 ACCTCCGGTAAGATAGTCGG AACATTTCAAGGACCACCCC 55.0 50.0 58.0 58.0 2103 bp 0.25 49.8
orf276_p5 CTCCGGTAAGATAGTCGGGT AACATTTCAAGGACCACCCC 55.0 50.0 58.0 58.0 2101 bp 0.25 49.8