Hotspot

atpF

Genome · 57784-58658 · 875 bp

Pi 0.192

MarkerSeek score 82.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 875
variable_sites: 277
pi: 0.192
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGCAATACCATCCCCAACTT	GTATTGCTCGTCAGCCTGAA	50.0	50.0	58.2	58.0	1425 bp	0.25	48.0
atpF_p2	CGCAATACCATCCCCAACTT	TTGGAATCGGTGCAAGAACA	50.0	45.0	58.2	58.0	1595 bp	0.25	47.9
atpF_p3	CGCAATACCATCCCCAACTT	ACTTGTTCCCGTACCCCTAA	50.0	50.0	58.2	58.0	1760 bp	0.25	47.9
atpF_p4	CGCAATACCATCCCCAACTT	TTACTTGTTCCCGTACCCCT	50.0	50.0	58.2	58.0	1762 bp	0.25	47.9
atpF_p5	CGCAATACCATCCCCAACTT	TCCCGTACCCCTAATGTTGT	50.0	50.0	58.2	58.0	1754 bp	0.25	47.7