Hotspot

accD

Genome · 77135-78160 · 1026 bp

Back to Codium

Pi 0.162
MarkerSeek score 81.70
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
Genome
length_bp
1026
variable_sites
269
pi
0.162
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 AATTGCTGGAACACCGACTT AATTCGACCGGTTGTTCCAA 45.0 45.0 58.0 58.0 2297 bp 0.25 49.6
accD_p2 ACCAACAACCCTCGGTTTAC AATTCGACCGGTTGTTCCAA 50.0 45.0 58.0 58.0 1845 bp 0.25 49.5
accD_p3 CCAACAACCCTCGGTTTACA AATTCGACCGGTTGTTCCAA 50.0 45.0 58.0 58.0 1844 bp 0.25 49.5
accD_p4 TCCATCCATTTGAGCTGCTC AATTCGACCGGTTGTTCCAA 50.0 45.0 58.0 58.0 2207 bp 0.25 49.4
accD_p5 ATCCATTTGAGCTGCTCCAG AATTCGACCGGTTGTTCCAA 50.0 45.0 57.9 58.0 2204 bp 0.25 49.3