Hotspot

orf107-petG

Genome · 54350-55709 · 1360 bp

Back to Codium

Pi 0.132
MarkerSeek score 83.00
Primer available yes
Label orf107-petG

Feature Metadata

label_name
orf107-petG
feature_type
igs
region
Genome
length_bp
1360
variable_sites
276
pi
0.132
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf107-petG_p1 AAAACGTCGCACTCGTGTAT CAGGAAGGAGATGTGTCTGC 45.0 55.0 58.2 58.0 2149 bp 0.25 48.0
orf107-petG_p2 AAAACGTCGCACTCGTGTAT CCGATCTTGATCAGGCAACA 45.0 50.0 58.2 58.0 1914 bp 0.25 48.0
orf107-petG_p3 AAAACGTCGCACTCGTGTAT CTTTCCGATCAGGTGGCTAC 45.0 55.0 58.2 58.1 1825 bp 0.25 47.7
orf107-petG_p4 AAAACGTCGCACTCGTGTAT AATCTCCGTTTCACGAGTGG 45.0 50.0 58.2 57.9 2033 bp 0.25 47.6
orf107-petG_p5 AAAACGTCGCACTCGTGTAT GGTCTTCCGCACAACCTAAA 45.0 50.0 58.2 58.1 2013 bp 0.25 47.3