Hotspot

ndhH

SSC · 122034-123215 · 1182 bp

Back to Cochlearia

Pi 0.253

MarkerSeek score 69.90

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 448
pi: 0.253
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TTTAACCGACTCGCACGTAG	GAAAACGTCAACGACTGCTG	50.0	50.0	58.0	58.0	80-1955 bp	0.75	81.7
ndhH_p2	GTTGTTGTATCCCTGCCGAT	GAAAACGTCAACGACTGCTG	50.0	50.0	58.0	58.0	80-1567 bp	0.75	81.7
ndhH_p3	ACTAATCGATTGGGCAGCAG	GAAAACGTCAACGACTGCTG	50.0	50.0	58.0	58.0	80-1896 bp	0.75	81.6
ndhH_p4	TTGAAGGATTCCCAATGGGC	GAAAACGTCAACGACTGCTG	50.0	50.0	58.1	58.0	80-1608 bp	0.75	80.9
ndhH_p5	AAGTAGTTTTGTCCCGTCCG	GAAAACGTCAACGACTGCTG	50.0	50.0	57.8	58.0	80-1635 bp	0.75	80.7