Hotspot

ndhF

SSC · 110022-112262 · 2241 bp

Back to Cochlearia

Pi 0.284
MarkerSeek score 80.90
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2241
variable_sites
955
pi
0.284
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGATGATGAATTCCACGTTCG TGAAATCCACATGAGAGCCC 47.6 50.0 57.4 57.6 80-2984 bp 0.75 65.9
ndhF_p2 GGATGATGAATTCCACGTTCG AAATCCACATGAGAGCCCTC 47.6 50.0 57.4 57.3 80-2982 bp 0.75 63.5
ndhF_p3 GGATGATGAATTCCACGTTCG GAAATCCACATGAGAGCCCT 47.6 50.0 57.4 57.3 80-2983 bp 0.75 63.5
ndhF_p4 GATGAATTCCACGTTCGAACA TGAAATCCACATGAGAGCCC 42.9 50.0 56.9 57.6 80-2980 bp 0.75 62.0
ndhF_p5 TGATGAATTCCACGTTCGAAC TGAAATCCACATGAGAGCCC 42.9 50.0 56.9 57.6 80-2981 bp 0.75 62.0