Hotspot

ndhE

SSC · 117807-118112 · 306 bp

Back to Cochlearia

Pi 0.271

MarkerSeek score 67.90

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 124
pi: 0.271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CCAGATCCAAAGAACGCGTA	CGGGGTTGTTTGGACTACAA	50.0	50.0	58.0	58.0	80-1383 bp	0.75	81.7
ndhE_p2	ATGCTTCGCGTTGTTTCATG	CGGGGTTGTTTGGACTACAA	45.0	50.0	58.0	58.0	80-1221 bp	0.75	81.5
ndhE_p3	CATGCTTCGCGTTGTTTCAT	CGGGGTTGTTTGGACTACAA	45.0	50.0	58.0	58.0	80-1222 bp	0.75	81.5
ndhE_p4	CGAACCGGCAGATATTGGAA	CGGGGTTGTTTGGACTACAA	50.0	50.0	58.1	58.0	80-1457 bp	0.75	81.3
ndhE_p5	CCAGATCCAAAGAACGCGTA	TGCGGCACAACTCCTTATTT	50.0	45.0	58.0	58.1	80-1569 bp	0.75	81.2