Hotspot

ccsA

SSC · 114439-115425 · 987 bp

Back to Cochlearia

Pi 0.248

MarkerSeek score 68.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 987
variable_sites: 367
pi: 0.248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCTAAAGCATCTCGGTTCGA	TTATCGATGGCTTCCCTTGC	50.0	50.0	58.1	58.0	80-1717 bp	0.75	81.1
ccsA_p2	GTTTTGAACAAGTTGCCGCT	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	80-1776 bp	0.75	81.1
ccsA_p3	AAGTTGCCGCTATGGTGAAA	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	80-1767 bp	0.75	81.0
ccsA_p4	TAGACACGCTGCTCTTAGGA	TTATCGATGGCTTCCCTTGC	50.0	50.0	57.9	58.0	80-1743 bp	0.75	80.7
ccsA_p5	AGTTGCCGCTATGGTGAAAT	TTATCGATGGCTTCCCTTGC	45.0	50.0	57.9	58.0	80-1766 bp	0.75	80.7