Hotspot

ndhG-ndhI

SSC · 118912-119272 · 361 bp

Back to Cochlearia

Pi 0.237

MarkerSeek score 66.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 128
pi: 0.237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TTGTAGTCCAAACAACCCCG	TCAACAAACCCTACGAGCTG	50.0	50.0	58.0	57.8	80-1192 bp	0.75	80.5
ndhG-ndhI_p2	TTGTAGTCCAAACAACCCCG	GTCAACAAACCCTACGAGCT	50.0	50.0	58.0	57.8	80-1193 bp	0.75	80.4
ndhG-ndhI_p3	AGAAGACCGGATCCCAGAAA	TCAACAAACCCTACGAGCTG	50.0	50.0	58.0	57.8	80-869 bp	0.75	80.3
ndhG-ndhI_p4	AGAAGACCGGATCCCAGAAA	GTCAACAAACCCTACGAGCT	50.0	50.0	58.0	57.8	80-870 bp	0.75	80.3
ndhG-ndhI_p5	ACTAGAAGACCGGATCCCAG	TCAACAAACCCTACGAGCTG	55.0	50.0	57.9	57.8	80-872 bp	0.75	80.1