Hotspot

psaC-ndhE

SSC · 117559-117806 · 248 bp

Back to Cochlearia

Pi 0.251

MarkerSeek score 66.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 248
variable_sites: 93
pi: 0.251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CCAGATCCAAAGAACGCGTA	CGGGGTTGTTTGGACTACAA	50.0	50.0	58.0	58.0	80-1383 bp	0.75	81.7
psaC-ndhE_p2	ATGCTTCGCGTTGTTTCATG	CGGGGTTGTTTGGACTACAA	45.0	50.0	58.0	58.0	80-1221 bp	0.75	81.5
psaC-ndhE_p3	CATGCTTCGCGTTGTTTCAT	CGGGGTTGTTTGGACTACAA	45.0	50.0	58.0	58.0	80-1222 bp	0.75	81.5
psaC-ndhE_p4	CGAACCGGCAGATATTGGAA	CGGGGTTGTTTGGACTACAA	50.0	50.0	58.1	58.0	80-1457 bp	0.75	81.3
psaC-ndhE_p5	GCATGCTCGAACACATTGAG	CGGGGTTGTTTGGACTACAA	50.0	50.0	57.8	58.0	80-1054 bp	0.75	80.4