Hotspot

ndhF-rpl32

SSC · 112263-113113 · 851 bp

Back to Cochlearia

Pi 0.242

MarkerSeek score 81.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 851
variable_sites: 309
pi: 0.242
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCCACATGCGTCGAAGATTT	TTTTTCCCCGTTTGGTTCCT	45.0	45.0	57.9	57.8	80-1229 bp	0.75	80.0
ndhF-rpl32_p2	GCAATAGGTCGTGTGAACCA	TTTTTCCCCGTTTGGTTCCT	50.0	45.0	58.2	57.8	80-1620 bp	0.75	79.1
ndhF-rpl32_p3	TGCAATAGGTCGTGTGAACC	TTTTTCCCCGTTTGGTTCCT	50.0	45.0	58.2	57.8	80-1621 bp	0.75	79.1
ndhF-rpl32_p4	GGTCGTGTGAACCAAAAACC	TTTTTCCCCGTTTGGTTCCT	50.0	45.0	57.8	57.8	80-1614 bp	0.75	79.1
ndhF-rpl32_p5	TGGTTGCTGTCGGAAAAAGT	TTTTTCCCCGTTTGGTTCCT	45.0	45.0	58.2	57.8	80-1208 bp	0.75	78.8