Hotspot

accD-psaI

LSC · 57941-58581 · 641 bp

Back to Cochlearia

Pi 0.00806

MarkerSeek score 65.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 10
pi: 0.00806
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGATGCAATTGTACCACGT	ATTGCAAGTGCCGGAAAAAC	45.0	45.0	57.8	58.1	810-880 bp	1.0	79.0
accD-psaI_p2	TGGATGCAATTGTACCACGT	CATTGCAAGTGCCGGAAAAA	45.0	45.0	57.8	58.1	811-881 bp	1.0	79.0
accD-psaI_p3	TGGATGCAATTGTACCACGT	AAAGAAGCCATTGCAAGTGC	45.0	45.0	57.8	57.8	819-889 bp	1.0	78.8
accD-psaI_p4	TGGATGCAATTGTACCACGT	TGCAAGTGCCGGAAAAACTA	45.0	45.0	57.8	58.3	808-878 bp	1.0	77.5
accD-psaI_p5	TGGATGCAATTGTACCACGT	GCAAGTGCCGGAAAAACTAG	45.0	50.0	57.8	57.7	807-877 bp	1.0	77.4