Hotspot

petD-rpoA

LSC · 89527-89727 · 201 bp

Back to Coccinia

Pi 0.02

MarkerSeek score 64.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 4
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGATTGGTACCGCAGTAGC	AGATTTTCGCATAGAAGACGT	50.0	38.1	58.0	55.4	352-355 bp	1.0	52.5
petD-rpoA_p2	TTGATTGGTACCGCAGTAGC	TCGCATAGAAGACGTAAAACA	50.0	38.1	58.0	55.4	346-349 bp	1.0	52.2
petD-rpoA_p3	AGCCCTTTGGTTGGGTATTG	AGATTTTCGCATAGAAGACGT	50.0	38.1	58.1	55.4	335-338 bp	1.0	52.2
petD-rpoA_p4	GGTTGGGTATTGGAGCAACA	AGATTTTCGCATAGAAGACGT	50.0	38.1	58.1	55.4	327-330 bp	1.0	52.1
petD-rpoA_p5	TGGTTGGGTATTGGAGCAAC	AGATTTTCGCATAGAAGACGT	50.0	38.1	58.1	55.4	328-331 bp	1.0	52.1