Hotspot

petA-psbJ

LSC · 74535-75538 · 1004 bp

Back to Coccinia

Pi 0.00805

MarkerSeek score 64.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 8
pi: 0.00805
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	CCTGTGATCGGTTTAGTCGG	45.0	55.0	58.0	58.1	1175-1182 bp	1.0	80.2
petA-psbJ_p2	ACGTGTACAAGGCCTTTTGT	TCCGGCTTAGGTTCATCTCT	45.0	50.0	58.0	57.8	1133-1140 bp	1.0	79.6
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	CCGGCTTAGGTTCATCTCTG	45.0	55.0	58.0	57.8	1132-1139 bp	1.0	79.0
petA-psbJ_p4	TCCATTACGTGTACAAGGCC	CCTGTGATCGGTTTAGTCGG	50.0	55.0	57.6	58.1	1181-1188 bp	1.0	77.5
petA-psbJ_p5	CCATTACGTGTACAAGGCCT	CCTGTGATCGGTTTAGTCGG	50.0	55.0	57.6	58.1	1180-1187 bp	1.0	77.4