Hotspot

psaI-ycf4

LSC · 71316-71718 · 403 bp

Back to Coccinia

Pi 0.0149

MarkerSeek score 56.00

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 6
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	ACGTTCTGATCGCCAATTCA	45.0	45.0	58.0	57.9	485-505 bp	1.0	80.2
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	546-566 bp	1.0	79.6
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	549-569 bp	1.0	79.6
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	ACGTTCTGATCGCCAATTCA	45.0	45.0	57.9	57.9	479-499 bp	1.0	79.6
psaI-ycf4_p5	GGCAATTGCAATGGCTTCTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	540-560 bp	1.0	79.0