Hotspot

trnS-rps4

LSC · 56613-56905 · 293 bp

Back to Coccinia

Pi 0.0239

MarkerSeek score 65.60

Primer available yes

Label trnS-rps4

Feature Metadata

label_name: trnS-rps4
feature_type: igs
region: LSC
length_bp: 293
variable_sites: 7
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-rps4_p1	CGGTTTCTGGGCCATAATCA	TGACTCTTCAGCCATTGCAA	50.0	45.0	57.9	57.7	523 bp	1.0	78.5
trnS-rps4_p2	CGGTTTCTGGGCCATAATCA	TTGACTCTTCAGCCATTGCA	50.0	45.0	57.9	57.7	524 bp	1.0	78.5
trnS-rps4_p3	AGGCTTTTGTTTACCGAGGG	TGACTCTTCAGCCATTGCAA	50.0	45.0	58.1	57.7	449 bp	1.0	78.3
trnS-rps4_p4	AGGCTTTTGTTTACCGAGGG	TTGACTCTTCAGCCATTGCA	50.0	45.0	58.1	57.7	450 bp	1.0	78.3
trnS-rps4_p5	TCGGTTTCTGGGCCATAATC	TGACTCTTCAGCCATTGCAA	50.0	45.0	57.7	57.7	524 bp	1.0	76.1