Hotspot

rpoB-trnC

LSC · 37190-38135 · 946 bp

Back to Coccinia

Pi 0.0161

MarkerSeek score 62.40

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 946
variable_sites: 15
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	CTCTCATTTCCACCCCCAAG	TGTTTGTTGATGAGGCGACA	55.0	45.0	57.9	58.0	1038-1050 bp	1.0	80.9
rpoB-trnC_p2	CTCTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1017-1029 bp	1.0	80.6
rpoB-trnC_p3	CTCTCATTTCCACCCCCAAG	TGGGGAAAAAGGGATTTGCA	55.0	45.0	57.9	57.9	1005-1017 bp	1.0	80.4
rpoB-trnC_p4	TCTCTCATTTCCACCCCCAA	TGTTTGTTGATGAGGCGACA	50.0	45.0	58.2	58.0	1039-1051 bp	1.0	80.0
rpoB-trnC_p5	TTCTCTCATTTCCACCCCCA	TGTTTGTTGATGAGGCGACA	50.0	45.0	58.2	58.0	1040-1052 bp	1.0	80.0