Hotspot

rps16-trnQ

LSC · 15422-16758 · 1337 bp

Back to Coccinia

Pi 0.0068
MarkerSeek score 63.70
Primer available yes
Label rps16-trnQ

Feature Metadata

label_name
rps16-trnQ
feature_type
igs
region
LSC
length_bp
1337
variable_sites
9
pi
0.0068
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1405-1410 bp 1.0 79.6
rps16-trnQ_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1416-1421 bp 1.0 79.1
rps16-trnQ_p3 AAGTCGCACGTTGCTTTCTA GACGACCAACCACTACCAAT 45.0 50.0 58.1 57.5 1501-1506 bp 1.0 76.4
rps16-trnQ_p4 TCATGTCCTTCAAGTCGCAC GACGACCAACCACTACCAAT 50.0 50.0 58.2 57.5 1512-1517 bp 1.0 75.8
rps16-trnQ_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1409-1414 bp 1.0 74.8