Hotspot

ycf1

IRb · 112415-114034 · 1620 bp

Back to Cnidoscolus

Pi 0.00682

MarkerSeek score 48.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1620
variable_sites: 11
pi: 0.00682
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	AATATGTAGGAAGCGGTCGC	55.0	50.0	58.0	57.8	2062-2091 bp	1.0	80.6
ycf1_p2	CCCGGAACTTCTTCGTAGTG	AATATGTAGGAAGCGGTCGC	55.0	50.0	58.0	57.8	1967-1996 bp	1.0	80.6
ycf1_p3	GTCTTCGTTCCATGCCTCAT	AATATGTAGGAAGCGGTCGC	50.0	50.0	58.0	57.8	1948-1977 bp	1.0	80.4
ycf1_p4	TCCCGGAACTTCTTCGTAGT	AATATGTAGGAAGCGGTCGC	50.0	50.0	58.1	57.8	1968-1997 bp	1.0	80.0
ycf1_p5	GAGCTACTGAGGAACAACGG	TACGAAATATGACGTGGCCC	55.0	50.0	58.0	57.8	2668-2697 bp	1.0	79.6