Hotspot

rpoC1

LSC · 20912-23763 · 2852 bp

Back to Cnidoscolus

Pi 0.0014

MarkerSeek score 51.70

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2852
variable_sites: 4
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TGGTCCTAGTTGAACCGAAG	TCCTTGTATCTGAGAAGAACTTTCA	50.0	36.0	56.9	57.7	2997-2998 bp	1.0	40.1
rpoC1_p2	ACTTAGCTTAGATCCCTATGA	TTGCTCGTTCGAGAACTACG	38.1	50.0	52.6	58.0	2992-2993 bp	1.0	40.1
rpoC1_p3	ACTTAGCTTAGATCCCTATGA	ACGATCTTTGGCTCTGGAAC	38.1	50.0	52.6	57.9	2975-2976 bp	1.0	40.1
rpoC1_p4	GGTCCTAGTTGAACCGAAGAG	TCCTTGTATCTGAGAAGAACTTTCA	52.4	36.0	57.8	57.7	2996-2997 bp	1.0	40.1
rpoC1_p5	TGGTCCTAGTTGAACCGAAG	AGGAAGGAAGCTTAATTGAAATGA	50.0	33.3	56.9	56.6	2965-2966 bp	1.0	40.1