Hotspot

psbE

LSC · 65972-66223 · 252 bp

Back to Cnidoscolus

Pi 0.00794

MarkerSeek score 48.40

Primer available yes

Label psbE

Feature Metadata

label_name: psbE
feature_type: gene
region: LSC
length_bp: 252
variable_sites: 2
pi: 0.00794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE_p1	ACGGTAGGTACAGCTAGTCC	ACAAGAATATGTGGAGCTCAGT	55.0	40.9	58.0	57.2	356 bp	1.0	57.4
psbE_p2	GGTAGGTACAGCTAGTCCGT	ACAAGAATATGTGGAGCTCAGT	55.0	40.9	58.0	57.2	354 bp	1.0	57.4
psbE_p3	GTCCGTGAACAGCTAACCAT	ACAAGAATATGTGGAGCTCAGT	50.0	40.9	57.9	57.2	340 bp	1.0	57.0
psbE_p4	AGCTAGTCCGTGAACAGCTA	ACAAGAATATGTGGAGCTCAGT	50.0	40.9	58.2	57.2	345 bp	1.0	56.5
psbE_p5	CGATCTATGGTCATTGGGCC	ACAAGAATATGTGGAGCTCAGT	55.0	40.9	58.2	57.2	296 bp	1.0	56.4