Hotspot

ndhK

LSC · 50570-51247 · 678 bp

Back to Cnidoscolus

Pi 0.00147

MarkerSeek score 59.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TTTCTCTCTCCCGCATTTTT	ATGCATGGAGAAAAGGAGCA	40.0	45.0	55.3	57.5	879 bp	1.0	54.0
ndhK_p2	TTTCTCTCTCCCGCATTTTT	TGCATGGAGAAAAGGAGCAT	40.0	45.0	55.3	57.5	878 bp	1.0	54.0
ndhK_p3	TTTTCTCTCTCCCGCATTTT	ATGCATGGAGAAAAGGAGCA	40.0	45.0	55.3	57.5	880 bp	1.0	54.0
ndhK_p4	TTTTCTCTCTCCCGCATTTT	TGCATGGAGAAAAGGAGCAT	40.0	45.0	55.3	57.5	879 bp	1.0	54.0
ndhK_p5	TTTTTCTCTCTCCCGCATTT	TGCATGGAGAAAAGGAGCAT	40.0	45.0	55.3	57.5	880 bp	1.0	54.0