Hotspot

ndhA

SSC · 123576-125784 · 2209 bp

Back to Cnidoscolus

Pi 0.000905

MarkerSeek score 45.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2209
variable_sites: 2
pi: 0.000905
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGCAGCTCGTATGGTTTGTT	ACCAATGCTACGAGCTTCTG	45.0	50.0	58.1	58.0	2868 bp	1.0	79.1
ndhA_p2	TTGCAGCTCGTATGGTTTGT	ACCAATGCTACGAGCTTCTG	45.0	50.0	58.1	58.0	2869 bp	1.0	79.1
ndhA_p3	TGCAGCTCGTATGGTTTGTT	TTAGAACGAGTTGAGGGGGT	45.0	50.0	58.1	58.0	2933 bp	1.0	79.1
ndhA_p4	TTGCAGCTCGTATGGTTTGT	TTAGAACGAGTTGAGGGGGT	45.0	50.0	58.1	58.0	2934 bp	1.0	79.1
ndhA_p5	TGCAGCTCGTATGGTTTGTT	AAGGGATTCGGAATGGAACG	45.0	50.0	58.1	58.0	2637 bp	1.0	78.9