Hotspot

ndhI-ndhA

SSC · 123495-123575 · 81 bp

Back to Cnidoscolus

Pi 0.0123
MarkerSeek score 59.60
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
81
variable_sites
1
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 TGCAGCTCGTATGGTTTGTT ACCTCTTTCCAACTTCTTTCACT 45.0 39.1 58.1 57.9 165 bp 1.0 54.7
ndhI-ndhA_p2 TTGCAGCTCGTATGGTTTGT ACCTCTTTCCAACTTCTTTCACT 45.0 39.1 58.1 57.9 166 bp 1.0 54.7
ndhI-ndhA_p3 ACCAATGTACCTTGCAGCTC ACCTCTTTCCAACTTCTTTCACT 50.0 39.1 58.2 57.9 177 bp 1.0 54.1
ndhI-ndhA_p4 GACCAATGTACCTTGCAGCT ACCTCTTTCCAACTTCTTTCACT 50.0 39.1 58.2 57.9 178 bp 1.0 54.1
ndhI-ndhA_p5 TCGTATGGTTTGTTGCCCAT ACCTCTTTCCAACTTCTTTCACT 45.0 39.1 57.8 57.9 159 bp 1.0 53.9