Hotspot

ndhF-rpl32

SSC · 116336-117300 · 965 bp

Back to Cnidoscolus

Pi 0.00208

MarkerSeek score 58.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 965
variable_sites: 2
pi: 0.00208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTTGTTGCTGCCGGAAAAAG	CGCTAACGAAAAAGCTTTCA	45.0	40.0	58.1	55.2	1171 bp	1.0	57.7
ndhF-rpl32_p2	AAAAAGCAGGAGTCCCACTC	CGCTAACGAAAAAGCTTTCA	50.0	40.0	57.7	55.2	1157 bp	1.0	56.0
ndhF-rpl32_p3	GAAAAAGCAGGAGTCCCACT	CGCTAACGAAAAAGCTTTCA	50.0	40.0	57.7	55.2	1158 bp	1.0	56.0
ndhF-rpl32_p4	TTTGTTGCTGCCGGAAAAAG	CGCTAACGAAAAAGCTTTCAA	45.0	38.1	58.1	56.0	1171 bp	1.0	55.9
ndhF-rpl32_p5	TTTTGTTGCTGCCGGAAAAA	CGCTAACGAAAAAGCTTTCA	40.0	40.0	57.6	55.2	1172 bp	1.0	55.2