Hotspot

ycf4-cemA

LSC · 61478-62426 · 949 bp

Back to Cnidoscolus

Pi 0.00211
MarkerSeek score 60.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
949
variable_sites
2
pi
0.00211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 GGGGCTATTCCTTTGACTCG AAAAAGAGATCCACCCGGG 55.0 52.6 58.0 57.0 1130 bp 1.0 64.0
ycf4-cemA_p2 AATTGAGCAAAAAGTCGCGG AAAAAGAGATCCACCCGGG 45.0 52.6 57.9 57.0 1083 bp 1.0 63.9
ycf4-cemA_p3 AGCAAAAAGTCGCGGAATTG AAAAAGAGATCCACCCGGG 45.0 52.6 57.9 57.0 1078 bp 1.0 63.9
ycf4-cemA_p4 TTGCGTGTACCGATTGAAGT AAAAAGAGATCCACCCGGG 45.0 52.6 57.8 57.0 1049 bp 1.0 63.1
ycf4-cemA_p5 GGGGCTATTCCTTTGACTCG GATCCACCCGGGCAAAAA 55.0 55.6 58.0 57.9 1123 bp 1.0 62.9