Hotspot

psbZ-trnG-GCC

LSC · 37153-37872 · 720 bp

Back to Cnidoscolus

Pi 0.00278

MarkerSeek score 60.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 2
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTCTTCTCCTGAGGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	876-882 bp	1.0	77.9
psbZ-trnG-GCC_p2	TGAGGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	867-873 bp	1.0	76.5
psbZ-trnG-GCC_p3	TCTTCTCCTGAGGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	875-881 bp	1.0	76.0
psbZ-trnG-GCC_p4	CTGAGGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.6	57.8	868-874 bp	1.0	75.9
psbZ-trnG-GCC_p5	CCTGAGGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.7	57.8	869-875 bp	1.0	72.9