Hotspot

rpoB-trnC-GCA

LSC · 27003-27994 · 992 bp

Back to Cnidoscolus

Pi 0.00202

MarkerSeek score 59.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 2
pi: 0.00202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTCCATTCCCGAG	CTGCGCCTTTTGTTGATCAG	55.0	50.0	58.0	58.0	1104 bp	1.0	79.7
rpoB-trnC-GCA_p2	CTCATTTCCATTCCCGAGCA	CTGCGCCTTTTGTTGATCAG	50.0	50.0	58.0	58.0	1102 bp	1.0	79.6
rpoB-trnC-GCA_p3	CCCTCATTTCCATTCCCGAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1077 bp	1.0	79.3
rpoB-trnC-GCA_p4	CTCATTTCCATTCCCGAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1075 bp	1.0	79.2
rpoB-trnC-GCA_p5	CCCTCATTTCCATTCCCGAG	TAAAGCTGCGCCTTTTGTTG	55.0	45.0	58.0	57.9	1109 bp	1.0	78.9