Hotspot

trnS-GCU-trnR-UCU

LSC · 8816-10679 · 1864 bp

Back to Cnidoscolus

Pi 0.00215

MarkerSeek score 59.60

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1864
variable_sites: 4
pi: 0.00215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1935-1967 bp	1.0	64.0
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1934-1966 bp	1.0	64.0
trnS-GCU-trnR-UCU_p3	TGCCAAAGTCATCACCGAAA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	2009-2041 bp	1.0	63.8
trnS-GCU-trnR-UCU_p4	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1941-1973 bp	1.0	63.3
trnS-GCU-trnR-UCU_p5	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1940-1972 bp	1.0	63.3