Hotspot

rps16-trnQ-UUG

LSC · 6259-7417 · 1159 bp

Back to Cnidoscolus

Pi 0.00173

MarkerSeek score 59.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 2
pi: 0.00173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1232-1241 bp	1.0	78.4
rps16-trnQ-UUG_p2	CCGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1247-1256 bp	1.0	76.6
rps16-trnQ-UUG_p3	TAAGAATCCACAGCCGATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1260-1269 bp	1.0	75.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1236-1245 bp	1.0	73.7
rps16-trnQ-UUG_p5	CGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.7	58.1	1246-1255 bp	1.0	73.6