Hotspot

ycf1

SSC · 125655-131134 · 5480 bp

Back to Cnidium

Pi 0.0106

MarkerSeek score 47.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5480
variable_sites: 58
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTTTCTTGGCGTTGCTGTT	GTTATCAAAAACGTTTTATTTCTG	45.0	25.0	57.8	52.0	299 bp	1.0	41.6
ycf1_p2	TCTTTTCTTGGCGTTGCTGT	GTTATCAAAAACGTTTTATTTCTG	45.0	25.0	58.6	52.0	300 bp	1.0	41.6
ycf1_p3	TTCTTGGCGTTGCTGTTTTT	GTTATCAAAAACGTTTTATTTCTG	40.0	25.0	57.3	52.0	296 bp	1.0	41.6
ycf1_p4	TTTCTTGGCGTTGCTGTTTT	GTTATCAAAAACGTTTTATTTCTG	40.0	25.0	57.3	52.0	297 bp	1.0	41.6
ycf1_p5	TTTTCTTGGCGTTGCTGTTT	GTTATCAAAAACGTTTTATTTCTG	40.0	25.0	57.3	52.0	298 bp	1.0	41.6