Hotspot

rps16

LSC · 4934-6031 · 1098 bp

Back to Cnidium

Pi 0.011

MarkerSeek score 65.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1098
variable_sites: 12
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCAATTTTGTTTGATTAGGACCA	TGTATCGACGGAACCAATGAC	30.4	47.6	54.7	58.1	1199-1206 bp	1.0	41.3
rps16_p2	TCAATTTTGTTTGATTAGGACCA	GTATCGACGGAACCAATGACT	30.4	47.6	54.7	57.8	1198-1205 bp	1.0	41.3
rps16_p3	TCAATTTTGTTTGATTAGGACCAAG	TGTATCGACGGAACCAATGAC	32.0	47.6	56.5	58.1	1199-1206 bp	1.0	41.3
rps16_p4	TCAATTTTGTTTGATTAGGACCA	TGTATCGACGGAACCAATGA	30.4	45.0	54.7	56.7	1199-1206 bp	1.0	41.3
rps16_p5	TCAATTTTGTTTGATTAGGACCAAG	GTATCGACGGAACCAATGACT	32.0	47.6	56.5	57.8	1198-1205 bp	1.0	41.3