Hotspot

rbcL

LSC · 56423-57850 · 1428 bp

Back to Cnidium

Pi 0.0112

MarkerSeek score 65.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 16
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1509 bp	1.0	70.3
rbcL_p2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.1	58.4	1510 bp	1.0	70.3
rbcL_p3	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.0	58.4	1557 bp	1.0	70.1
rbcL_p4	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTAATTGCA	45.0	45.0	57.0	58.4	1558 bp	1.0	70.1
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	47.6	45.0	57.8	58.4	1510 bp	1.0	68.2