Hotspot

trnL-CAA-ycf2

IRa · 146402-147549 · 1148 bp

Back to Cnidium

Pi 0.103

MarkerSeek score 80.80

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 1148
variable_sites: 98
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	GTTCGAGTCCTCTTCAAGGC	ATGGCTTTTCCCGGATGAAA	55.0	45.0	58.3	57.8	447-1236 bp	1.0	87.9
trnL-CAA-ycf2_p2	GTTCGAGTCCTCTTCAAGGC	TGGCTTTTCCCGGATGAAAT	55.0	45.0	58.3	57.8	446-1235 bp	1.0	87.9
trnL-CAA-ycf2_p3	GTTCGAGTCCTCTTCAAGGC	AAGATGGCTTTTCCCGGATG	55.0	50.0	58.3	58.2	450-1239 bp	1.0	87.9
trnL-CAA-ycf2_p4	GTTCGAGTCCTCTTCAAGGC	GATGGCTTTTCCCGGATGAA	55.0	50.0	58.3	58.2	448-1237 bp	1.0	87.8
trnL-CAA-ycf2_p5	GTTCGAGTCCTCTTCAAGGC	AAAGATGGCTTTTCCCGGAT	55.0	45.0	58.3	57.5	451-1240 bp	1.0	85.5