Hotspot

accD-psaI

LSC · 59948-60351 · 404 bp

Back to Cnidium

Pi 0.0149

MarkerSeek score 63.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 6
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.8	57.9	527-547 bp	1.0	74.1
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	50.0	45.0	58.8	58.2	517-537 bp	1.0	73.7
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	52.6	45.0	58.1	57.9	527-547 bp	1.0	71.7
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	42.9	45.0	58.1	57.9	525-545 bp	1.0	71.6
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	AAAGAAGCCATTGCCATTGC	50.0	45.0	56.9	57.9	538-558 bp	1.0	71.4