Hotspot

trnT-GGU-psbD

LSC · 32357-33793 · 1437 bp

Back to Cnidium

Pi 0.0126

MarkerSeek score 66.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1437
variable_sites: 18
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	50.0	50.0	58.1	58.1	1620-1637 bp	1.0	80.3
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1616-1633 bp	1.0	80.1
trnT-GGU-psbD_p3	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	50.0	55.0	58.1	57.8	1627-1644 bp	1.0	79.5
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	50.0	55.0	57.9	57.8	1623-1640 bp	1.0	79.2
trnT-GGU-psbD_p5	AAGGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	50.0	50.0	58.1	58.2	1628-1645 bp	1.0	79.1