Hotspot

trnE-UUC-trnT-GGU

LSC · 31535-32284 · 750 bp

Back to Cnidium

Pi 0.0242

MarkerSeek score 59.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 18
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGGAGTCAGAAGGGGATACT	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	1549-1637 bp	1.0	80.8
trnE-UUC-trnT-GGU_p2	CGGAGTCAGAAGGGGATACT	TGGTCCATTAAGCTGCGATC	55.0	50.0	57.9	58.0	2240-2346 bp	1.0	80.6
trnE-UUC-trnT-GGU_p3	CGGAGTCAGAAGGGGATACT	TGGTGATTCCAGAGACGACT	55.0	50.0	57.9	58.1	1821-1909 bp	1.0	80.2
trnE-UUC-trnT-GGU_p4	ATGTGCGCTTGGGAAACATA	GAACCGATGACTTACGCCTT	45.0	50.0	58.2	58.0	1449-1537 bp	1.0	80.1
trnE-UUC-trnT-GGU_p5	TATGTGCGCTTGGGAAACAT	GAACCGATGACTTACGCCTT	45.0	50.0	58.2	58.0	1450-1538 bp	1.0	80.1