Hotspot

psbM-trnD-GUC

LSC · 30472-31108 · 637 bp

Back to Cnidium

Pi 0.00646

MarkerSeek score 65.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 4
pi: 0.00646
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.6	58.5	748-802 bp	1.0	75.3
psbM-trnD-GUC_p2	AGGATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	50.0	50.0	57.6	57.0	749-803 bp	1.0	71.1
psbM-trnD-GUC_p3	AGGATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	50.0	52.6	57.6	57.8	747-801 bp	1.0	69.3
psbM-trnD-GUC_p4	GGAACTAGGATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.7	58.5	754-808 bp	1.0	67.8
psbM-trnD-GUC_p5	AGGATGAACAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	50.0	47.6	57.6	58.5	750-804 bp	1.0	67.2