Hotspot

trnK-UUU-rps16

LSC · 4232-4933 · 702 bp

Back to Cnidium

Pi 0.0228
MarkerSeek score 59.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
702
variable_sites
16
pi
0.0228
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG AAAGGCAGAGGTTTTTAAGT 50.0 35.0 58.0 52.9 802-811 bp 1.0 41.6
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AAAGGCAGAGGTTTTTAAGT 55.0 35.0 57.9 52.9 819-828 bp 1.0 41.6
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AAAGGCAGAGGTTTTTAAGT 55.0 35.0 57.9 52.9 818-827 bp 1.0 41.6
trnK-UUU-rps16_p4 TCCCTCGCTTCATCCAAATG AAAAGGCAGAGGTTTTTAAGT 50.0 33.3 58.0 53.8 803-812 bp 1.0 41.6
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC AAAAGGCAGAGGTTTTTAAGT 55.0 33.3 57.9 53.8 819-828 bp 1.0 41.6