Hotspot

ycf1

SSC · 126908-132295 · 5388 bp

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Pi 0.0061
MarkerSeek score 39.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5388
variable_sites
82
pi
0.0061
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTGGGAAAAACCAAGGTTCT GAGAATTCTCCATTTCTGTATTAGA 42.9 32.0 57.3 54.4 201-255 bp 1.0 42.5
ycf1_p2 TCTGGGAAAAACCAAGGTTCT AGAATTCTCCATTTCTGTATTAGA 42.9 29.2 57.3 53.2 200-254 bp 1.0 42.5
ycf1_p3 ACGCATCAAAAAGTAGCACA GAGAATTCTCCATTTCTGTATTAGA 40.0 32.0 56.3 54.4 224-278 bp 1.0 42.3
ycf1_p4 ACGCATCAAAAAGTAGCACA AGAATTCTCCATTTCTGTATTAGA 40.0 29.2 56.3 53.2 223-277 bp 1.0 42.3
ycf1_p5 ACGCATCAAAAAGTAGCACAA GAGAATTCTCCATTTCTGTATTAGA 38.1 32.0 57.0 54.4 224-278 bp 1.0 42.3