Hotspot

atpF

LSC · 12321-13750 · 1430 bp

Pi 0.00508

MarkerSeek score 40.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1430
variable_sites: 20
pi: 0.00508
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGCTCCTGGAAAGTAGTCAC	CCTTCCCGTCCTTCGTTTAG	55.0	55.0	58.0	58.0	2415-2449 bp	1.0	83.2
atpF_p2	CGCTCCTGGAAAGTAGTCAC	CCGGGAAGGACTGATTTGAG	55.0	55.0	58.0	58.0	2467-2501 bp	1.0	82.9
atpF_p3	CGCTCCTGGAAAGTAGTCAC	CGGGAAGGACTGATTTGAGG	55.0	55.0	58.0	58.0	2466-2500 bp	1.0	82.9
atpF_p4	CGCTCCTGGAAAGTAGTCAC	CTCATTGCCGTTTGCTTCTG	55.0	50.0	58.0	58.0	2540-2574 bp	1.0	82.8
atpF_p5	CCGAGATTCAGAAGCGGAAA	CCTTCCCGTCCTTCGTTTAG	50.0	55.0	58.0	58.0	2152-2186 bp	0.571429	65.7