Hotspot

petA-psbJ

LSC · 65326-66455 · 1130 bp

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Pi 0.0151
MarkerSeek score 56.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1130
variable_sites
40
pi
0.0151
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 57.8 58.3 1263-1271 bp 1.0 79.2
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC CATATTCCGGGTTGGGTTCA 50.0 50.0 57.8 57.6 1259-1267 bp 1.0 78.2
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC GTTCATATTCCGGGTTGGGT 50.0 50.0 57.8 57.6 1262-1270 bp 1.0 78.2
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC CGGTTCATATTCCGGGTTGG 50.0 55.0 57.8 58.7 1264-1272 bp 1.0 76.1
petA-psbJ_p5 GGCCTTTTGTTCTTCTTGGC TATTCCGGGTTGGGTTCATC 50.0 50.0 57.8 57.3 1257-1265 bp 1.0 75.9