Hotspot

ycf4-cemA

LSC · 62658-63455 · 798 bp

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Pi 0.00854
MarkerSeek score 54.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
798
variable_sites
17
pi
0.00854
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA ATGAGAGAGAGACCCACCAG 45.0 55.0 57.9 57.8 878-967 bp 1.0 83.0
ycf4-cemA_p2 CTTGCGCGTACCAATTGAAG ATGAGAGAGAGACCCACCAG 50.0 55.0 58.1 57.8 880-969 bp 1.0 82.7
ycf4-cemA_p3 TGCGCGTACCAATTGAAGTA GGGAGGGAAGTCAATGCTTT 45.0 50.0 57.9 57.8 831-920 bp 1.0 82.7
ycf4-cemA_p4 CTTGCGCGTACCAATTGAAG GGGAGGGAAGTCAATGCTTT 50.0 50.0 58.1 57.8 833-922 bp 1.0 82.4
ycf4-cemA_p5 GGAACCATTCCCTTGACTCG ATGAGAGAGAGACCCACCAG 55.0 55.0 58.3 57.8 960-1049 bp 1.0 81.1