Hotspot

trnC-GCA-petN

LSC · 28406-29462 · 1057 bp

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Pi 0.0114
MarkerSeek score 43.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1057
variable_sites
29
pi
0.0114
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 CCAGTTCAAATCCGGGTGTC TTAAAGCAGCCCAAGCAAGA 55.0 45.0 58.8 58.0 1115-1122 bp 1.0 75.4
trnC-GCA-petN_p2 CCAGTTCAAATCCGGGTGTC CAGCCCAAGCAAGACTTACT 55.0 50.0 58.8 57.8 1109-1116 bp 1.0 73.9
trnC-GCA-petN_p3 CAGTTCAAATCCGGGTGTCG TTAAAGCAGCCCAAGCAAGA 55.0 45.0 59.2 58.0 1114-1121 bp 1.0 72.5
trnC-GCA-petN_p4 AGTTCAAATCCGGGTGTCG TTAAAGCAGCCCAAGCAAGA 52.6 45.0 57.8 58.0 1113-1120 bp 1.0 72.3
trnC-GCA-petN_p5 CCAGTTCAAATCCGGGTGT TTAAAGCAGCCCAAGCAAGA 52.6 45.0 57.7 58.0 1115-1122 bp 1.0 71.4