Hotspot

rpl32-trnL-UAG

SSC · 112470-112945 · 476 bp

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Pi 0.0958
MarkerSeek score 80.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
476
variable_sites
43
pi
0.0958
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGAAAGCTTTTTCATTGGCACA TCGAACCGAGATGCTTTAGC 36.4 50.0 57.9 58.1 618 bp 0.5 42.3
rpl32-trnL-UAG_p2 AAAAGCGCACTTCGATATCA TCGAACCGAGATGCTTTAGC 40.0 50.0 55.9 58.1 690 bp 0.5 42.3
rpl32-trnL-UAG_p3 AAAGCGCACTTCGATATCAA TCGAACCGAGATGCTTTAGC 40.0 50.0 55.9 58.1 689 bp 0.5 42.3
rpl32-trnL-UAG_p4 AAGCGCACTTCGATATCAAA TCGAACCGAGATGCTTTAGC 40.0 50.0 55.9 58.1 688 bp 0.5 42.3
rpl32-trnL-UAG_p5 AGCGCACTTCGATATCAAAA TCGAACCGAGATGCTTTAGC 40.0 50.0 55.9 58.1 687 bp 0.5 42.3